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« Previous Entries Next Entries »genetic manipulation in fish
9:54 AMjuly 08
Sex Ratio Manipulation
- For aquaculture or stocking, it may be desirable to use only one sex due to differences in growth rate or characteristics concerned with maturity and reproduction,
- Sex ratio can be manipulated either directly (using steroid hormones to alter the sex ratio in fry during sexual differentiation) or indirectly (manipulating the sex […]
genetic manipulation in practice
9:52 AMjuly 08
Sperm:
- Strip milt from males,
- dilute sperm in 0.85% NaCl (1/200 dilution and then 1/4000 dilution),
- count all of the sperm in 5 blocks (5×5) of hemacytometer,
- calculate sperm concentration (10^10)=(total count/80)x4000Ax4000Bx1000C (A : inverse of dilution, B : volume of hemacytometer cell = 1/4000 uL, C : to convert from concentration per uL […]
fish mating system
9:49 AMjuly 07
…….under corrected…….