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The Effective Combination of Different Extender and Cryoprotectant on Sperm Cryopreservation of Nilem (Indonesian Sharkminnow, Osteochilus hasseltii Valenciennes, 1842)

A. Sunarma, D.W.B. Hastuti, D.M. Saleh, Y. Sistina
paper presented at Fourth Annual National Seminar of Result of Fisheries and Marine Research, Yogya, July 28, 2007

Abstract
Cryopreservation technology of fish sperm has been developed to prolong gamete viability. Combination of two extenders (ringer or glucose) and two cryoprotectans (DMSO or methanol) on three concentrations have studied to sperm cryopreservation of nilem. Sperm was diluted in extender at the ratio of 1:9 then cryoprotectant was added at 5%, 10% or 15% (v/v) concentrations. Samples were stored in 0,5 mL straws, equilibrated at temperature 4 – 5 ^oC for 20 minutes, vaporized at 3 cm above surface liquid nitrogen for 3 minutes and then plunged into liquid nitrogen, where they were stored for 1 weeks. Sperm was thawed at temperature 39 – 40 ^oC for 10 – 15 sec. and was used to fertilize 100 – 200 eggs per straw. The highest percentage of post-thawed sperm was combination ringer and DMSO 10% (87,50 ± 5,00%) and the lowest was combination ringer and methanol 5% (23,75 ± 4,79%). The highest hatching rate fertilized by post-thawed sperm was combination ringer and DMSO 15% (54,98 ± 28,61%) and the lowest was combination glucose and DMSO 15% (6,54 ± 3,32%). The study showed fish sperm cryopreservation with nilem as a representative model could be developed to extend on other Cyprinidae.

Keywords: cryopreservation, sperm, nilem fish

paper in Indonesian